Species identification of aphids (Insecta: Hemiptera: Aphididae) through DNA barcodes.

نویسندگان

  • R G Foottit
  • H E L Maw
  • C D VON Dohlen
  • P D N Hebert
چکیده

A 658-bp fragment of mitochondrial DNA from the 5' region of the mitochondrial cytochrome c oxidase 1 (COI) gene has been adopted as the standard DNA barcode region for animal life. In this study, we test its effectiveness in the discrimination of over 300 species of aphids from more than 130 genera. Most (96%) species were well differentiated, and sequence variation within species was low, averaging just 0.2%. Despite the complex life cycles and parthenogenetic reproduction of aphids, DNA barcodes are an effective tool for identification.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Barcoding aphids (Hemiptera: Aphididae) of the Korean Peninsula: updating the global data set.

DNA barcode (mitochondrial COI) sequences are provided for species identification of aphids from the Korean Peninsula. Most (98%) of the 154 species had distinct COI sequences (average 0.05% intraspecific pairwise divergence) relative to the degree of sequence divergence among species (average value 5.84%). For species in common with other regions, barcodes for Korean samples fell near or withi...

متن کامل

DNA Barcoding and the Associated PhylAphidB@se Website for the Identification of European Aphids (Insecta: Hemiptera: Aphididae)

UNLABELLED Aphids constitute a diverse group of plant-feeding insects and are among the most important crop pests in temperate regions. Their morphological identification is time-consuming and requires specific knowledge, training and skills that may take years to acquire. We assessed the advantages and limits of DNA barcoding with the standard COI barcode fragment for the identification of Eur...

متن کامل

Molecular and morphological evaluation of the aphid genus Hyalopterus Koch (Insecta: Hemiptera: Aphididae), with a description of a new species

Aphids in the genus Hyalopterus Koch (Hemiptera: Aphididae) are pests of stone fruit trees in the genus Prunus globally, causing damage directly through feeding as well as transmission of plant viruses. Despite their status as cosmopolitan pests, the genus is poorly understood, with current taxonomy recognizing two, likely paraphyletic, species: Hyalopterus pruni (Koch) and Hyalopterus amygdali...

متن کامل

Cryptic diversity of the subfamily Calaphidinae (Hemiptera: Aphididae) revealed by comprehensive DNA barcoding

Aphids are a species rich group comprising many important pests. However, species identification can be very difficult for aphids due to their morphological ambiguity. DNA barcoding has been widely adopted for rapid and reliable species identification as well as cryptic species detection. In this study, we investigated cryptic diversity in the subfamily Calaphidinae (Hemiptera: Aphididae) based...

متن کامل

Wing Morphometry Helps Diagnose Cryptic Species and Resurrect Mindarus pinicolus (Hemiptera: Aphididae)

Wing venation, two-dimensional and with easily recognized reference points at vein junctions, presents an opportunity for the development of automated insect identiÞcation. Using a suite of continuous characters, I investigated the use of wing morphometry for computerized insect identiÞcation of cryptic species of the aphid genusMindarus. A priori groups were determined using cytochrome oxidase...

متن کامل

ذخیره در منابع من

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Molecular ecology resources

دوره 8 6  شماره 

صفحات  -

تاریخ انتشار 2008